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ying-li-python
fasta-fix
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"""
Welcome! The purpose of this script is to help fix the spacing issue
when a sequence is downloaded as a FASTA file from a public database.
The result will give you the entire sequence in a single line and stored
in a new fasta file.
Please see READNE.md for detailed instructions.
"""
# import dependencies
import os
# open and read FASTA file
fastafile = open("FlyBase_YGMHKX.fasta", 'r')
# assign variables to store header and sequence result
fasta_header = ''
fixed_sequence = ''
# create for loop to iterate every line of the file
for line in fastafile:
# strips line
line = line.strip()
# for header that starts with the symbol >
if line[0] == ">":
# create a variable that stores the header information with the symbol > at the start
fasta_header = ">" + line[1:]
# for lines that do not start with the symbol >
else:
# concatenate lines and store sequence to variable
fixed_sequence = fixed_sequence+line
# print results in terminal
print(f'{fasta_header}\n{fixed_sequence}')
# write results in output file
with open("output.fasta", "w") as output_file:
output_file.write(f'{fasta_header}\n{fixed_sequence}')
# close files
fastafile.close()
output_file.close()
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